Molecular Biology

Department of Medical Microbiology & Immunology Research Laboratory

MOLECULAR BIOLOGY

To equip learners with a basic understanding of molecular biology techniques in current use and the role they play in Medical Microbiology & Immunology

Learning Objectives

At the end of this course, the student should be able to:

  1. Describe the structure and function of nucleic acids and general principles of genetic information flow
  2. Describe current techniques used in molecular biology
  3. Apply molecular biology techniques in other fields of medical microbiology

Content

DNA: Structure and function, DNA:- Molecular biology techniques; RNA: - Structure and function, RNA: - Molecular biology techniques;

Proteins: - structure and synthesis; Impact of molecular methods on clinical bacteriology, clinical bacteriology, clinical virology and immunology. Plasmid Analysis; Genomic DNA digestion and

Ribotyping, DNA Amplification; - general concepts and methods, Primed PCR methods for studying bacterial diseases (mycobacterium tuberculosis and other infections caused by mycobacterium species, streptococci, meningococcal diseases). PCR methods for studying viral diseases (Hapea, HIV, HBV, HCV, HPV, SARS-CoV-2). Molecular approaches of Enzyme Technology and tissue engineering, Molecular approaches for the detection and identification of drug resistance. Molecular Biology of parasitic diseases (malaria, trypanosomiasis, Leishmaisis, Pneumocystis)